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Journal: International Journal of Molecular Sciences
Article Title: 1-Hydroxypyrene Promotes Bladder Cancer Progression and Tumor-Associated Immunosuppression via MIF
doi: 10.3390/ijms27177778
Figure Lengend Snippet: MIF mediates 1-OHP-induced tumor-associated immunosuppression and PI3K/AKT/mTOR pathway activation. ( A , B ) Correlation analysis showing the associations of MIF expression with CD74 and CD44, respectively. ( C , D ) Flow cytometric analysis of CD74 and CD44 expression in EJ cells after the indicated treatments. ( E , F ) Schematic illustration of PBMC isolation, CD8 + T-cell sorting, and co-culture with bladder cancer cells. ( G – L ) Flow cytometric analysis of Ki67, GZMB, and IFNG expression in CD8 + T cells after co-culture with differently treated EJ cells. ( M ) GSEA plot showing enrichment of the HALLMARK_MTORC1_SIGNALING pathway. ( N ) Correlation analysis showing a positive association between MIF expression and PI3K/AKT pathway activity. ( O , P ) Western blot analysis of PI3K/AKT/mTOR pathway activation in EJ and T24 cells under the indicated treatments. ns, not significant; * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
Article Snippet: The antibodies included ACTIN (Abclonal, Cat. No. AC026), mTOR (Abclonal, Cat. No. A2445), p-mTOR (Abclonal, Cat. No. AP0094),
Techniques: Activation Assay, Expressing, Isolation, FACS, Co-Culture Assay, Activity Assay, Western Blot